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Marti, R. / Mieszkin, Sophie / Solecki, O. / Pourcher, A. -m. / Hervio-heath, Dominique / Gourmelon, Michele - 2011Aims: The aim is to evaluate the dynamic of Bacteroides-Prevotella and Bacillus-Streptococcus-Lactobacillus populations originating from pig manure and the persistence of pig-associated markers belonging to these groups according to temperature and oxygen. Methods and Results: River water was...Contributeur (s):
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Solecki, O. / Jeanneau, L. / Jarde, E. / Gourmelon, Michele / Marin, Charlotte / Pourcher, A. M. - 2011Natural seawater and freshwater microcosms inoculated with pig manure were set up to determine the persistence of pig faecal microbial and chemical markers in these two types of surface water. The concentrations of Lactobacillus amylovorus, the Bacteroidales Pig-2-Bac 165 rRNA genetic marker, five...Contributeur (s):
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Alzieu, Claude / Andral, Bruno / Bassoulet, Philippe / Gourmelon, Michele / Le Quillec, Régis / L'Yavanc, Jacky / Quiniou, Francoise - 1999Les traitements in situ des sédiments, ou biodragages, consistent à disperser à la surface ou à injecter dans le sédiment des produits pulvérulents sur lesquels sont fixées des bactéries capables de dégrader les matières organiques. Peu de données expérimentales sont disponibles quant à l'...Contributeur (s):
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Mieszkin, Sophie / Furet, Jean-pierre / Corthier, Gerard / Pommepuy, Monique / Le Saux, Jean-claude / Bougeard, Morgane / Hervio Heath, Dominique / Gourmelon, Michele - 2009The microbiological quality of coastal waters and shellfish harvesting areas in Brittany (France) can be affected by faecal pollutions from human activities and animal breeding (especially pigs and cattle). To discriminate among faecal pollution of human and animal origin, a library-independent...Contributeur (s):
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Gourmelon, Michele / Caprais, Marie-paule / Mieszkin, Sophie / Marti, Romain / Wery, Nathalie / Jarde, E. / Derrien, Marc / Jadas-hecart, A. / Communal, P. Y. / Jaffrezic, A. / Pourcher, A. M. - 2010The microbiological quality of coastal or river waters can be affected by faecal pollution from human or animal sources. An efficient MST (Microbial Source Tracking) toolbox consisting of several host-specific markers would therefore be valuable for identifying the origin of the faecal pollution in...Contributeur (s):
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Gourmelon, Michele / Caprais, Marie-paule / Le Mennec, Cecile / Mieszkin, Sophie / Ponthoreau, C. / Gendronneau, M. - 2010Faecal contamination sources were identified in coastal areas around the Guerande-Atlantique peninsula using two microbial source tracking (MST) methods: (i) Bacteroidales host-specific 16S rRNA gene markers measured by real-time PCR and (ii) F-specific bacteriophage (FRNAPH) genotyping. Both...Contributeur (s):
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Furet, Jean-pierre / Firmesse, Olivier / Gourmelon, Michele / Bridonneau, Chantal / Tap, Julien / Mondot, Stanislas / Dore, Joel / Corthier, Gerard - 2009Pollution of the environment by human and animal faecal pollution affects the safety of shellfish, drinking water and recreational beaches. To pinpoint the origin of contaminations, it is essential to define the differences between human microbiota and that of farm animals. A strategy based on real...Contributeur (s):
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Aims: The aims of this study were to evaluate the host-specific distribution of Bacteroidales 16S rRNA gene sequences from human- and animal-related effluents and faeces, and to define a ruminant-specific marker. Methods and Results: Bacteroidales 16S rRNA gene clone libraries were constructed from...Contributeur (s):
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The microbiological quality of coastal or river water can be affected by fecal contamination from human or animal sources. To discriminate pig fecal pollution from other pollution, a library-independent microbial source tracking method targeting Bacteroidales host-specific 16S rRNA gene markers by...Contributeur (s):
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Gawler, A / Beecher, J / Brandao, J / Carroll, N / Falcao, L / Gourmelon, Michele / Masterson, B / Nunes, B / Porter, J / Rince, A / Rodrigues, R / Thorp, M / Walters, J / Meijer, W - 2007The recent implementation of the Revised Bathing Water Directive in the European Union has highlighted the need for development of effective methods to differentiate between sources of faecal contamination. It had previously been shown that amplification of 16S rRNA genes of host-specific...Contributeur (s):
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Gourmelon, Michele / Caprais, Marie-paule / Segura, Raphael / Le Mennec, Cecile / Lozach, Solen / Piriou, Jean-yves / Rince, A - 2007In order to identify the origin of the fecal contamination observed in French estuaries, two library-independent microbial source tracking (MST) methods were selected: (i) Bacteroidales host-specific 16S rRNA gene markers and (ii) F-specific RNA bacteriophage genotyping. The specificity of the...Contributeur (s):
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Pommepuy, Monique / Hervio Heath, Dominique / Caprais, Marie-paule / Gourmelon, Michele / Le Saux, Jean-claude / Le Guyader, Soizick - 2006Introduction : "The occurrence of pathogenic microorganisms in seawater or in shellfish could exist anytime sewage from human or animal origin would be discharged to the coast" (Metcalf, 1982). According to the diseases occurring in the human population or in animals, pathogens might be...Contributeur (s):
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Alzieu, Claude / Abarnou, Alain / Bassoullet, Philippe / Boutier, Bernard / Chiffoleau, Jean-francois / Crenn, Isabelle / Derrien, Annick / Erard-le Denn, Evelyne / Gourmelon, Michele / Guiliaud, Jean-françois / Silva Jacinto, Ricardo / Le Cann, Pierre / Le Guyader, Françoise / Le Hir, Pierre / L'Yavanc, Jacky / Mauvais, Jean-louis / Menard, Dominique / Michel, Pierre / Monbet, Yves / Pommepuy, Monique / Quiniou, Francoise - 1999Port activities require continuous or occasional dredging of considerable quantities of sediments : approximately 50 million m3 along the French coastline. A large proportion of the dredgings is authorized for dumping at sea in geographically delimited areas and following an impact evaluation of...Contributeur (s):
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Gourmelon, Michele / Montet, M / Lozach, Solen / Le Mennec, Cecile / Pommepuy, Monique / Beutin, L / Vernozy Rozand, C - 2006Aims: This study was carried out to evaluate the presence of Shiga toxin-producing Escherichia coli (STEC) and E. coli O157:H7 in shellfish from French coastal environments. Methods and Results: Shellfish were collected in six growing areas or natural beds (B category) and nonfarming areas (D...Contributeur (s):
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Troussellier, Marc / Bonnefont, Jean-luc / Courties, Claude / Derrien, Annick / Dupray, Elizabeth / Gauthier, Michel / Gourmelon, Michele / Joux, Fabien / Lebaron, Philippe / Martin, Yvan / Pommepuy, Monique - 1998The effects of different environmental factors (nutrient deprivation, hyperosmotic shock, exposure to light) on enteric bacteria which have been transferred into the marine environment, have been studied experimentally (microcosms) by considering demographic, physiological and genetic responses in...Contributeur (s):
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