Methodological approaches for histamine quantification using derivatization by chloroethylnitrosourea and ELISA measurement. Part I: Optimisation of derivated histamine detection with coated-plates using optimal design

Using derivatization by chloroethylnitrosourea (CENU) a new strategy was used for raising antibodies directed against hapten (<300 Da) and the quantification of these haptens by ELISA. After raising antibodies directed against histamine, they were characterized and used for ELISA measurements. The development of a quantitative method needs an optimization of both detection and derivatization step. Experimental design methodology has been applied to optimize the conditions of the detection of the derivatized histamine. Methods and results were closely related.